Journal: Renal Failure

Article Title: TNF-α-mediated podocyte injury via the apoptotic death receptor pathway in a mouse model of IgA nephropathy

doi: 10.1080/0886022X.2022.2079527

Figure Lengend Snippet: PCR primer sequence.

Article Snippet: The membranes were blocked with 5% skimmed milk for 2 h, and incubated with specific primary antibodies overnight at 4 °C: Podocin (1:500; cat. no. P0372; Sigma-Aldrich; Merck KGaA), nephrin (1:1,000; cat. no. BAF3159; R&D Systems, Inc.), GAPDH (1:1,000; cat. no. 2118; Cell Signaling Technology, Inc.), TNF-α (1:2,000; cat. no. ab9739; Abcam), caspase-8 (1:1,000; cat. no. ab2590; Abcam), caspase-3 (1:1,000; cat. no. ab184787; Abcam) and TNFR1 (1:1,000; cat. no. sc-8436; Santa Cruz Biotechnology, Inc.).

Techniques: Sequencing

Journal: Renal Failure

Article Title: TNF-α-mediated podocyte injury via the apoptotic death receptor pathway in a mouse model of IgA nephropathy

doi: 10.1080/0886022X.2022.2079527

Figure Lengend Snippet: Proteins and mRNA expressions of podocin and nephrin are significantly reduced in IgAN mouse kidneys. (A) Western blotting results and (B) relative protein expression of nephrin and podocin. (C) Transcription levels of nephrin and podocin ( n = 3). Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01 vs. control group. IgAN, IgA nephropathy.

Article Snippet: The membranes were blocked with 5% skimmed milk for 2 h, and incubated with specific primary antibodies overnight at 4 °C: Podocin (1:500; cat. no. P0372; Sigma-Aldrich; Merck KGaA), nephrin (1:1,000; cat. no. BAF3159; R&D Systems, Inc.), GAPDH (1:1,000; cat. no. 2118; Cell Signaling Technology, Inc.), TNF-α (1:2,000; cat. no. ab9739; Abcam), caspase-8 (1:1,000; cat. no. ab2590; Abcam), caspase-3 (1:1,000; cat. no. ab184787; Abcam) and TNFR1 (1:1,000; cat. no. sc-8436; Santa Cruz Biotechnology, Inc.).

Techniques: Western Blot, Expressing, Control

Journal: Renal Failure

Article Title: TNF-α-mediated podocyte injury via the apoptotic death receptor pathway in a mouse model of IgA nephropathy

doi: 10.1080/0886022X.2022.2079527

Figure Lengend Snippet: PCR primer sequence.

Article Snippet: Double immunofluorescence staining for nephrin (1:250; cat. no. BAF3159; R&D Systems, Inc.) and cleaved caspase-3 (1:400; cat. no. 9661; Cell Signaling Technology, Inc.) was carried out to examine the location of apoptosis in kidney glomerular paraffin sections.

Techniques: Sequencing

Journal: Renal Failure

Article Title: TNF-α-mediated podocyte injury via the apoptotic death receptor pathway in a mouse model of IgA nephropathy

doi: 10.1080/0886022X.2022.2079527

Figure Lengend Snippet: Proteins and mRNA expressions of podocin and nephrin are significantly reduced in IgAN mouse kidneys. (A) Western blotting results and (B) relative protein expression of nephrin and podocin. (C) Transcription levels of nephrin and podocin ( n = 3). Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01 vs. control group. IgAN, IgA nephropathy.

Article Snippet: Double immunofluorescence staining for nephrin (1:250; cat. no. BAF3159; R&D Systems, Inc.) and cleaved caspase-3 (1:400; cat. no. 9661; Cell Signaling Technology, Inc.) was carried out to examine the location of apoptosis in kidney glomerular paraffin sections.

Techniques: Western Blot, Expressing, Control

Journal: Renal Failure

Article Title: TNF-α-mediated podocyte injury via the apoptotic death receptor pathway in a mouse model of IgA nephropathy

doi: 10.1080/0886022X.2022.2079527

Figure Lengend Snippet: Colocalization of nephrin and cleaved caspase-3 in podocyte apoptosis in IgAN mice. (A) Double immunofluorescence was used to determine the expression of nephrin and cleaved caspase-3 in podocytes (magnification, x400). (B) Double immunofluorescence flux rate. Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01 vs. control group. IgAN, IgA nephropathy.

Article Snippet: Double immunofluorescence staining for nephrin (1:250; cat. no. BAF3159; R&D Systems, Inc.) and cleaved caspase-3 (1:400; cat. no. 9661; Cell Signaling Technology, Inc.) was carried out to examine the location of apoptosis in kidney glomerular paraffin sections.

Techniques: Immunofluorescence, Expressing, Control